PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
免疫原
Flag tag
验证实验
验证实验
ELISA, WB, IF
建议稀释比例
WB: 1:1000-1:4000; IF: 1:10-1:100
验证图片
Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03154(Flag tag antibody) at dilution of 1:1000
Immunofluorescent analysis of Transfected HEK-293 cells using FNab03154 (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
背景介绍
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag(3xDYKDDDDKT) sequence and recognizes the(3x)DYKDDDDK peptide and detects FLAG-tagged proteins.