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PCNA antibody

别称:Proliferating cell nuclear antigen antibody, Cyclin antibody
货号:FNab06216反应性:Human, Mouse, Rat, Rabbit, pig, monkey, zebrafish, hamster
宿主:Rabbit验证实验:ELISA, WB, IHC, IP
克隆性:polyclonal亚型:IgG
¥1500
规格:
货期: 三个工作日+顺丰快递时长
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产品名称
PCNA antibody
货号
FNab06216
规格
100μg
状态
liquid
纯化方法
Immunogen affinity purified
纯度
≥95% as determined by SDS-PAGE
克隆性
polyclonal
亚型
IgG
存储
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
免疫原
proliferating cell nuclear antigen
别称
Proliferating cell nuclear antigen antibody, Cyclin antibody
UniProt ID
P12004
分子量
38 kDa
验证实验
验证实验
ELISA, WB, IHC, IP
建议稀释比例
WB: 1:500 - 1:2000; IHC: 1:50 - 1:500
验证图片
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06216(PCNA antibody) at dilution of 1:2000
IP Result of anti-PCNA (IP:FNab06216, 3ug; Detection:FNab06216 1:1500) with MCF-7 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human stomach cancer using FNab06216(PCNA antibody) at dilution of 1:50
背景介绍
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
期刊名:
Research Square
作者:
桂林医科大学附属桂东人民医院
检测样本:
cultured KGN cells and rat ovarian tissue
发布日期:
2024-03-08
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