产品
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HIF1a antibody
¥1900
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- HIF1a antibody
- 货号
- FNab09940
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- protein A+G purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- monoclonal
- 亚型
- IgG1
- Clone ID
- 5H12
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
- 别称
- BHLHE78 antibody, MOP1 antibody, PASD8 antibody
- UniProt ID
- Q16665
- 分子量
- 120 kDa
验证实验
- 验证实验
- ELISA, IF, WB
- 建议稀释比例
- WB: 1:1000-1:10000; IF: 1:50-1:200
验证图片
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab09940(HIF1a Antibody) at dilution of 1:5000
Immunofluorescent analysis of ( -20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using FNab09940 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L)
- 背景介绍
- Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
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