产品
本公司产品仅供体外研究使用，不用于临床诊断
BrdU antibody
¥1900
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- BrdU antibody
- 货号
- FNab00952
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Protein A+G purification
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- monoclonal
- 亚型
- IgG2a
- Clone ID
- 9E8
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- compound
- 别称
- 5-bromo-2-deoxyuridine antibody, BrDu antibody, BrdUrd antibody, Bromodeoxyuridine antibody, BUdR antibody
验证实验
- 验证实验
- ELISA, IF, IHC
- 建议稀释比例
- IHC: 1:200-1:1000; IF: 1:50-1:500
验证图片
Immunohistochemistry of paraffin-embedded mouse spleen tissue slide(with BrdU feeding) using FNab00952(BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using FNab00952 ( BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- 背景介绍
- Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
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