MCF7 cells were subjected to SDS PAGE followed by western blot with FNab03890(Histone-H3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab03890(Histone-H3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HEK-293 cells using FNab03890 (Histone-H3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
背景介绍
HIST2H3A,histone cluster 2, H3a.It is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. HIST2H3A is Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.