产品
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MYC tag antibody
¥1900
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- MYC tag antibody
- 货号
- FNab05456
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Protein A+G purification
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- monoclonal
- 亚型
- IgG2a
- Clone ID
- 0G0
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- MYC-tag
- 别称
- myc tag antibody
验证实验
- 验证实验
- ELISA, WB, IP, IF
- 建议稀释比例
- WB: 1:2000-1:20000; IP: 1:2000-1:20000; IF: 1:10-1:100
验证图片
Myc-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab05456(MYC tag antibody) at dilution of 1:10000
IP Result of anti MYC tag (IP: FNab05456, 4ug; Detection: FNab05456 1:5000) with Transfected HEK-293 cells lysate 1000ug.
- 背景介绍
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.
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