产品
本公司产品仅供体外研究使用，不用于临床诊断
USP28 antibody
¥1900
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- USP28 antibody
- 货号
- FNab09322
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- ubiquitin specific peptidase 28
- 别称
- Deubiquitinating enzyme 28 antibody, KIAA1515 antibody, Ubiquitin thiolesterase 28 antibody, USP28 antibody
- UniProt ID
- Q96RU2
- 分子量
- 130 kDa
验证实验
- 验证实验
- ELISA, WB, IHC, IP
- 建议稀释比例
- WB: 1:500-1:5000; IP: 1：200-1：2000; IHC: 1：20-1：200
验证图片
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09322(USP28 antibody) at dilution of 1:1000
IP Result of anti-USP28 (IP:FNab09322, 4ug; Detection:FNab09322 1:600) with HeLa cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab09322(USP28 antibody) at dilution of 1:50
- 背景介绍
- Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome(APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7(FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7(FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) in a subset of colorectal cancers(CRC) cells(PubMed:24623306).
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