HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09344(USP9X antibody) at dilution of 1:500
IP Result of anti-USP9X (IP:FNab09344, 5ug; Detection:FNab09344 1:500) with HEK-293 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab09344(USP9X Antibody) at dilution of 1:50
背景介绍
Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'-and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Involved in axonal growth and neuronal cell migration.