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ZO-1 antibody

分类：

研究方向：

别称：	Tight junction protein 1 antibody, Tight junction protein ZO 1 antibody, TJP1 antibody, ZO 1 antibody, ZO1 antibody, ZO-1 antibody, Zona occludens protein 1 antibody, Zonula occludens protein 1 antibody
货号：	FNab09751	反应性：	Human, Mouse, Rat
宿主：	Rabbit	验证实验：	ELISA, WB, IHC, FC, IF, IP
克隆性：	polyclonal	亚型：	IgG

说明书

¥2100

规格：

货期：三个工作日+顺丰快递时长

产品名称: ZO-1 antibody
货号: FNab09751
规格: 100μg
状态: liquid
纯化方法: Immunogen affinity purified
纯度: ≥95% as determined by SDS-PAGE
克隆性: polyclonal
亚型: IgG
存储: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

免疫原信息

免疫原: tight junction protein 1(zona occludens 1)
别称: Tight junction protein 1 antibody, Tight junction protein ZO 1 antibody, TJP1 antibody, ZO 1 antibody, ZO1 antibody, ZO-1 antibody, Zona occludens protein 1 antibody, Zonula occludens protein 1 antibody
UniProt ID: Q07157
分子量: 230kd

验证实验

验证图片

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09751(ZO1 antibody) at dilution of 1:500

IP result of anti-ZO-1(IP:FNab09751, 4ug; Detection:FNab09751 1:500) with HEK-293 cells lysate 2320 ug

Immunohistochemistry of paraffin-embedded human kidney using FNab09751(ZO1 antibody) at dilution of 1:100

Immunofluorescent analysis of MCF7 cells using FNab09751(ZO-1 Antibody) at dilution of 1:100

背景介绍: Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).