产品
本公司产品仅供体外研究使用，不用于临床诊断
PCNA antibody
¥750
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- PCNA antibody
- 货号
- FNab06217
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Protein A+G purification
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- monoclonal
- 亚型
- IgG1
- Clone ID
- 8F9
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- proliferating cell nuclear antigen
- 别称
- Proliferating cell nuclear antigen antibody, Cyclin antibody
- UniProt ID
- P12004
- 分子量
- 38 kDa
验证实验
- 验证实验
- ELISA, WB, IHC, FC, IP
- 建议稀释比例
- WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500
验证图片
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06217(PCNA Antibody) at dilution of 1:10000
IP Result of anti-PCNA (IP:FNab06217, 4ug; Detection:FNab06217 1:300) with HepG2 cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab06217(PCNA antibody) at dilution of 1:800
Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using FNab06217 (PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
- 背景介绍
- Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
抗体定制服务
抗体定制服务
