产品
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p65/RELA antibody
¥1750
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- p65/RELA antibody
- 货号
- FNab06089
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- v-rel reticuloendotheliosis viral oncogene homolog A(avian)
- 别称
- NFKB 3 antibody, NFKB p65 antibody, NFKB3 antibody, p65 antibody, RELA antibody, Transcription factor p65 antibody
- UniProt ID
- Q04206
- 分子量
- 65 kDa
验证实验
- 验证实验
- ELISA, IHC, IF, IP, WB, FC
- 建议稀释比例
- WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:10-1:100
验证图片
HeLa cells were subjected to SDS PAGE followed by western blot with FNab06089(p65 antibody) at dilution of 1:1000
IP Result of anti-p65 (IP:FNab06089, 3ug; Detection:FNab06089 1:1000) with HeLa cells lysate 5000ug.
Immunohistochemistry of paraffin-embedded human liver cancer using FNab06089(p65 antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FNab06089(RELA antibody)at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- 背景介绍
- Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
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