产品
本公司产品仅供体外研究使用，不用于临床诊断
YY1 antibody
¥750
规格：
货期：
三个工作日+顺丰快递时长
- 产品名称
- YY1 antibody
- 货号
- FNab10027
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- YY1 transcription factor
- 别称
- Transcriptional repressor protein YY1|Delta transcription factor|INO80 complex subunit S|NF-E1|Yin and yang 1 (YY-1)|YY1|INO80S antibody
- UniProt ID
- P25490
- 分子量
- 60 kDa, 75 kDa
验证实验
- 验证实验
- ELISA, WB, IHC
- 建议稀释比例
- WB: 1:500-1:2000; IHC: 1:50-1:500
验证图片
A549 cells were subjected to SDS PAGE followed by western blot with FNab10027(YY1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using FNab10027(YY1 antibody) at dilution of 1:100
- 背景介绍
- Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein(BMP)-mediated cardiac-specific gene expression(PubMed:15329343). Binds to SMAD binding elements(SBEs)(5'-GTCT/AGAC-3') within BMP response element(BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures(Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.
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