产品
本公司产品仅供体外研究使用，不用于临床诊断
AREG antibody
| 规格 | 价格 |
|---|---|
| 100μg | RMB ¥1750 |
| 50μg | RMB ¥1050 |
货期：
三个工作日+顺丰快递时长
- 产品名称
- AREG antibody
- 货号
- FNab00374
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- amphiregulin
- 别称
- Amphiregulin (AR)|Colorectum cell-derived growth factor (CRDGF)|AREG|AREGB|SDGF antibody
- UniProt ID
- P15514
- 分子量
- 43 kDa
验证实验
- 验证实验
- ELISA, IF, WB, IHC,IP
- 建议稀释比例
- WB: 1:200-1:2000; IHC: 1:50-1:500; IP: 1:1000-1:10000; IF: 1:10-1:100
验证图片
MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab00374(AREG Antibody) at dilution of 1:600
IP Result of anti-Amphiregulin (IP:FNab00374, 4ug; Detection:FNab00374 1:2000) with HepG2 cells lysate 3600ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab00374(AREG Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- 背景介绍
- Amphiregulin(AREG) is one of the ligands of the epidermal growth factor receptor(EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms(PMID: 9642297).
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