产品
本公司产品仅供体外研究使用，不用于临床诊断
- 产品名称
- BRCA1 antibody
- 货号
- FNab00943
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- breast cancer 1, early onset
- 别称
- Breast cancer type 1 susceptibility protein|RING finger protein 53|RING-type E3 ubiquitin transferase BRCA1|BRCA1|RNF53 antibody
- UniProt ID
- P38398
- 分子量
- 85-95 kDa
验证实验
- 验证实验
- ELISA, IHC, WB
- 建议稀释比例
- WB: 1:500-1:2000; IHC: 1:20-1:200
验证图片
human testis tissue were subjected to SDS PAGE followed by western blot with FNab00943(BRCA1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using FNab00943(BRCA1 antibody) at dilution of 1:100
- 背景介绍
- E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair(HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator(PubMed:20160719).
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