Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01539(CDCA2 antibody) at dilution of 1:500
IP Result of anti-CDCA2 (IP:FNab01539, 4ug; Detection:FNab01539 1:800) with Jurkat cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human gastric carcinoma tissue slide using FNab01539(CDCA2 Antibody) at dilution of 1:100.Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min.
背景介绍
Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit(PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.