CXCL12 antibody

抗体20ug一口价
别称:Stromal cell-derived factor 1 (SDF-1 antibody, hSDF-1)|C-X-C motif chemokine 12|Intercrine reduced in hepatomas (IRH antibody, hIRH)|Pre-B cell growth-stimulating factor (PBSF)|SDF-1-beta(3-72)|SDF-1-alpha(3-67)|CXCL12|SDF1|SDF1A|SDF1B antibody
货号:FNab02093反应性:Human, Mouse
宿主:Rabbit验证实验:ELISA, IHC
克隆性:polyclonal亚型:IgG
规格 价格
100μg RMB ¥1900
50μg RMB ¥1140
20μg RMB ¥570
货期: 三个工作日+顺丰快递时长
  • 产品介绍
  • 实验结果
  • 实验条件
  • 常见问题
产品名称
CXCL12 antibody
货号
FNab02093
规格
100μg
状态
liquid
纯化方法
Immunogen affinity purified
纯度
≥95% as determined by SDS-PAGE
克隆性
polyclonal
亚型
IgG
存储
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
免疫原
chemokine(C-X-C motif) ligand 12(stromal cell-derived factor 1)
别称
Stromal cell-derived factor 1 (SDF-1 antibody, hSDF-1)|C-X-C motif chemokine 12|Intercrine reduced in hepatomas (IRH antibody, hIRH)|Pre-B cell growth-stimulating factor (PBSF)|SDF-1-beta(3-72)|SDF-1-alpha(3-67)|CXCL12|SDF1|SDF1A|SDF1B antibody
UniProt ID
P48061
验证实验
验证实验
ELISA, IHC
建议稀释比例
IHC: 1:20-1:200
验证图片
Immunohistochemistry of paraffin-embedded human liver cancer tissue using FNab02093(CXCL12 antibody) at dilution of 1:50
背景介绍
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
抗体可以回收利用几次?

一般抗体不推荐回收利用,抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。

FineTest公司也做过一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定。

注:我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存,效价高的抗体可至少保存1周,重复利用3次左右。

FineTest公司抗体buffer成分是什么?

FineTest抗体成分一般是PBS含proclin300或sodium azide、BSA、50%甘油;也有一些是PBS含proclin300或sodium azide,50%甘油。防腐剂proclin300或sodium azide是常用的防腐剂,广泛应用于实验室和工业中。

FineTest公司抗体的保存温度和时间?

大部分抗体是放在-20℃保存,直标流式抗体需要放在2-8℃保存,质保期一年,质保期内抗体出现售后,可进行退货或换货。

一般抗体超过一年质保期仍旧可以使用,我们可以提供技术支持服务。

FineTest公司抗体使用需要稀释吗?用什么溶液稀释?

除了直标流式抗体为即用型,不用稀释。其他抗体一般为浓缩型,使用时需要按照说明书建议稀释比例稀释。

不同实验使用的稀释液不同,用PBST, TBST, 抗体封闭液等常用抗体稀释液均可。

免疫组化实验抗体的修复方式?

常用修复液:Tris-EDTA缓冲液(Tris-EDTA Buffer, pH 9.0);柠檬酸盐缓冲液(Citrate Buffer, pH 6.0)

热修复:

方法1:沸水浴修复,将盛有修复液和玻片的烧杯置于沸水浴环境,保持外部沸腾状态15min,自然冷却至室温;

方法2:微波修复,将盛有修复液和玻片的烧杯置于微波炉中,高火5min,停火3min,中火5min,自然冷却至室温。

如何选择二抗?

(1)二抗是和一抗反应的,所以二抗必须是抗一抗宿主物种,比如一抗来源于兔,对应二抗则选择抗兔二抗,比如羊抗兔或驴抗兔。

(2)根据实验类型选择二抗偶联物,比如做ELISA, 蛋白质印迹,IHC实验,常用酶联二抗,如HRP,AP等标记。而免疫荧光实验,流式细胞术实验中,则用荧光蛋白或染料标记,比如FITC,Cy3等。

产品对比