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EIF4A3 antibody
| 规格 | 价格 |
|---|---|
| 100μg | RMB ¥1900 |
| 50μg | RMB ¥1140 |
货期：
三个工作日+顺丰快递时长
- 产品名称
- EIF4A3 antibody
- 货号
- FNab02718
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- eukaryotic translation initiation factor 4A, isoform 3
- 别称
- Eukaryotic initiation factor 4A-III (eIF-4A-III antibody, eIF4A-III)|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD box protein 48|Eukaryotic initiation factor 4A-like NUK-34|Eukaryotic translation initiation factor 4A isoform 3|Nuclear matrix protein 265 (NMP 265 antibody, hNMP 265)|Eukaryotic initiation factor 4A-III antibody, N-terminally processed|EIF4A3|DDX48|KIAA0111 antibody
- UniProt ID
- P38919
- 分子量
- 47 kDa
验证实验
- 验证实验
- ELISA, WB, IP
- 建议稀释比例
- WB: 1:500-1:2000; IP: 1:200-1:2000
验证图片
A549 cells were subjected to SDS PAGE followed by western blot with FNab02718(EIF4A3 antibody) at dilution of 1:600
IP Result of anti-EIF4A3 (IP:FNab02718, 3ug; Detection:FNab02718 1:1000) with mouse heart tissue lysate 5000ug.
- 背景介绍
- ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
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