HeLa cells were subjected to SDS PAGE followed by western blot with FNab02847(ERK1/2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab02847(ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunofluorescent analysis of HepG2 cells using FNab02847(ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
背景介绍
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.