HepG2 cells were subjected to SDS PAGE followed by western blot with FNab03009(FANCI antibody) at dilution of 1:500
IP Result of anti-FANCI (IP:FNab03009, 4ug; Detection:FNab03009 1:1000) with Jurkat cells lysate 2400ug.
背景介绍
Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links(ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA(ssDNA) and double-stranded DNA(dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.