产品
本公司产品仅供体外研究使用，不用于临床诊断
MAPKAPK2 antibody
| 规格 | 价格 |
|---|---|
| 100μg | RMB ¥1500 |
| 50μg | RMB ¥900 |
| 20μg | RMB ¥450 |
货期：
三个工作日+顺丰快递时长
- 产品名称
- MAPKAPK2 antibody
- 货号
- FNab04998
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- mitogen-activated protein kinase-activated protein kinase 2
- 别称
- MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2 antibody, MAPKAP kinase 2 antibody, MAPKAP-K2 antibody, MAPKAPK-2 antibody, MK-2 antibody, MK2)|MAPKAPK2 antibody
- UniProt ID
- P49137
- 分子量
- 47-50 kDa
验证实验
- 验证实验
- ELISA, WB, IHC
- 建议稀释比例
- WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500
验证图片
HeLa cells were subjected to SDS PAGE followed by western blot with FNab04998(MAPKAPK2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab04998(MAPKAPK2 Antibody) at dilution of 1:50
- 背景介绍
- Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein(sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor(TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements(AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites(PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway(TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
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