HeLa cells were subjected to SDS PAGE followed by western blot with FNab05252(MNAT1 antibody) at dilution of 1:500
IP Result of anti-MNAT1 (IP:FNab05252, 4ug; Detection:FNab05252 1:500) with HeLa cells lysate 1200ug.
背景介绍
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase(CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain(CTD) of its large subunit(POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.