产品
本公司产品仅供体外研究使用,不用于临床诊断
- 产品介绍
- 相关文献
- 产品名称
- MYC-tag antibody
- 货号
- FNab05462
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- MYC-tag
- 别称
- myc tag antibody
验证实验
- 验证实验
- ELISA, WB, IP, IF
- 建议稀释比例
- WB: 1:1000-1:8000; IP: 1:500-1:2000 ; IF: 1:10-1:100
验证图片
Myc-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab05462(MYC tag antibody) at dilution of 1:4000
IP Result of anti-MYC-tag (IP: FNab05462, 4ug; Detection: FNab05462 1:1000) with Transfected HEK-293 cells lysate 300ug.
- 背景介绍
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.
IF: 6.543
- 期刊名:
- Oxidative Medicine and Cellular Longevity
- 发布日期:
- 2021-09-10
- 引用产品: