hela lysates were subjected to SDS PAGE followed by western blot with FNab09800(Vinculin Antibody) at dilution of 1:5000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09800(Vinculin Antibody) at dilution of 1:200
Immunofluorescent analysis of ( 4% PFA) fixed Hela cells using FNab09800 (vinculin antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Mouse IgG(H+L)
背景介绍
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.