Various lysates were subjected to SDS PAGE followed by western blot with FNab09833(PDI antibody) at dilution of 1:50000
Immunohistochemistry of paraffin-embedded human liver tissue slide using FNab09833( PDI Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab09833 (PDI antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
背景介绍
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).