产品
本公司产品仅供体外研究使用，不用于临床诊断
RAF1 antibody
| 规格 | 价格 |
|---|---|
| 100μg | RMB ¥1500 |
| 50μg | RMB ¥900 |
| 20μg | RMB ¥450 |
货期：
三个工作日+顺丰快递时长
- 产品名称
- RAF1 antibody
- 货号
- FNab10383
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- v-raf-1 murine leukemia viral oncogene homolog 1
- 别称
- RAF proto-oncogene serine/threonine-protein kinase|Proto-oncogene c-RAF (cRaf)|Raf-1|RAF1|RAF antibody
- UniProt ID
- P04049
- 分子量
- 76 kDa
验证实验
- 验证实验
- ELISA, WB, IHC
- 建议稀释比例
- WB: 1:500-1:2000; IHC: 1:20-1:200
验证图片
Rat skeletal muscle were subjected to SDS PAGE followed by western blot with FNab10383( RAF1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat kidney tissue slide using FNab10383(RAF1 Antibody) at dilution of 1:50
- 背景介绍
- Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase(MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases(MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases(MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1(on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility(ROCK2), apoptosis(MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis(RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin(OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
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