产品
本公司产品仅供体外研究使用，不用于临床诊断
PCNA antibody
| 规格 | 价格 |
|---|---|
| 100μg | RMB ¥1500 |
| 50μg | RMB ¥900 |
| 20μg | RMB ¥450 |
货期：
三个工作日+顺丰快递时长
- 产品名称
- PCNA antibody
- 货号
- FNab06216
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- proliferating cell nuclear antigen
- 别称
- Proliferating cell nuclear antigen (PCNA)|Cyclin|PCNA antibody
- UniProt ID
- P12004
- 分子量
- 38 kDa
验证实验
- 验证实验
- ELISA, WB, IHC, IP
- 建议稀释比例
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:500
验证图片
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06216(PCNA antibody) at dilution of 1:2000
IP Result of anti-PCNA (IP:FNab06216, 3ug; Detection:FNab06216 1:1500) with MCF-7 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human stomach cancer using FNab06216(PCNA antibody) at dilution of 1:50
- 背景介绍
- Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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