产品
本公司产品仅供体外研究使用，不用于临床诊断
VIM antibody
¥1900
规格：
货期：
三个工作日+顺丰&京东快递时长
- 产品名称
- VIM antibody
- 货号
- FNab09409
- 规格
- 100μg
- 状态
- liquid
- 纯化方法
- Immunogen affinity purified
- 纯度
- ≥95% as determined by SDS-PAGE
- 克隆性
- polyclonal
- 亚型
- IgG
- 存储
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
免疫原信息
- 免疫原
- vimentin
- 别称
- Vimentin|VIM antibody
- UniProt ID
- P08670
- 分子量
- 55 kDa
验证实验
- 验证实验
- ELISA, WB, IHC, IF
- 建议稀释比例
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
验证图片
Hela cells were subjected to SDS PAGE followed by western blot with FNab09409(VIM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary cancer using FNab09409(VIM antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using FNab09409(VIM antibody) at 1:25 dilution?
- 背景介绍
- Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
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